Manufactured by:Boster Bio
ABMIUM Verified™RUO

Anti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4

Primary Antibody supplied by Boster Bio

SKU:
ABM22681-1
Product type
Primary Antibodies
TargetSWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
ApplicationsELISA, Flow Cytometry, IHC, IHC-P, WB
Species / reactivityHuman, Mouse, Rat
PurityNo cross reactivity with other proteins.

Manufacturer provenance

Manufactured by:
Boster Bio
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Anti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4 is a research-use antibody from Boster Bio for studying SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4) catalog # M00223-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Transcription activator BRG1 also known as ATP-dependent helicase SMARCA4 is a protein that in humans is encoded by the SMARCA4 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

Product information

Key facts & specifications
Also known as
ATP dependent helicase SMARCA4; BAF190; BAF190A; BRG1; BRG1 associated factor 190A; hSNF2b; Protein brahma homolog 1; Protein BRG 1; SMARCA4; SMARCA4/BRG1; SNF2; SNF2 BETA; SNF2B; SNF2L4; SNF2LB; SWI2; Transcription activator BRG1
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: 3F4
Isotype
Mouse IgG1
Cross-reactivity
No cross-reactivity with other proteins.
Dilution range
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Immunogen
E. coli-derived human BRG1 recombinant protein (Position: Q555-E763).
Observed band
181 kDa
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification
Immunogen affinity purified.
Form / buffer
Lyophilized
Sequence fragment
E. coli-derived human BRG1 recombinant protein (Position: Q555-E763).
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
M00223-1
Gene symbol
SMARCA4
Gene ID
6597
UniProt accession
P51532
Storage, stability & shipping
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Storage warning
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Compliance & documents
CE marked
No
Datasheet
Open datasheet
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Boster Bio and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Boster Bio
£328.00