Manufactured by:ELK Biotechnology
RUO

ABHD7 rabbit pAb

ABHD7 rabbit pAb from ELK Biotechnology

SKU:
ABM105531
Product type
Primary Antibody
TargetABHD7
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

ABHD7 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ABHD7 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The α/β hydrolase superfamily comprise diverse members that are involved in important biochemical processes and related to various diseases. They have unrelated sequences, various substrates, and different kinds of catalytic activities, yet they share the same canonical α/β hydrolase fold, which consists of an eight-stranded parallel α/β structure. They are also characterized by a catalytic triad composed of a histidine, an acid and a nucleophile. Members of this superfamily are often drug targets for treating diseases, such as diabetes, Alzheimer's disease, obesity and blood clotting disorders. The Ab hydrolase domain containing (ABHD) gene subfamily is comprised of 15 mostly uncharacterized members, most of which utilize a serine nucleophile to form the G-X-S-X-G nucleophile elbow. ABHD7 is a 362 amino acid single-pass type II membrane protein that belongs to the AB hydrolase superfamily and the Epoxide hydrolase family.

Additional antibody information

Specificity ABHD7 Polyclonal Antibody detects endogenous levels of ABHD7 protein.
Validation evidence captions Western Blot analysis of various cells using ABHD7 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from HT-29 cells, using ABHD7 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using ABHD7 antibody.
Key facts & specifications
Research areas
>>Chemical carcinogenesis - receptor activation, >>Chemical carcinogenesis - reactive oxygen species
Also known as
EPHX4; ABHD7; EPHXRP; Epoxide hydrolase 4; Abhydrolase domain-containing protein 7; Epoxide hydrolase-related protein
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human ABHD7. AA range:101-150
Observed band
42kD
Cellular localization
Membrane ; Single-pass type II membrane protein .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 101-150
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES1574
UniProt entry
Q8IUS5
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00