Manufactured by:ELK Biotechnology
RUO

Rat TGFa(Transforming Growth Factor Alpha) Microsample ELISA Kit

Rat TGFa(Transforming Growth Factor Alpha) Microsample ELISA Kit from ELK Biotechnology

SKU:
ABM60005
Product type
ELISA Kits
TargetTGFa
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Rat TGFa(Transforming Growth Factor Alpha) Microsample ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring TGFa in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TGFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TGFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TGFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TGFa in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include TGF Alpha; ETGF; TGF-A; Protransforming Growth Factor alpha; EGF-Like TGF.

Key facts & specifications
Research areas
Cytokine, Tumor immunity, Infection immunity
Also known as
TGF Alpha; ETGF; TGF-A; Protransforming Growth Factor alpha; EGF-Like TGF
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
3.2 pg/mL
Detection range
7.82-500 pg/mL
Plate format
96-well pre-coated
Form / buffer
Pre-coated microplate kit
Quantity per pack
48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSerumPlasmaTissue homogenatesCell lysatesCell culture supernatantsOther biological fluids
Sample compatibility
Identifiers & database references
Supplier SKU / catalogue number
ELK2464MS
UniProt entry
P01134
Storage, stability & shipping
Storage conditions
Store according to the conditions stated in the product manual.
Long-term storage
Store according to the conditions stated in the product manual.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

TGFAtransforming growth factor alpha

View target guide

TGFA identifies transforming growth factor alpha in Homo sapiens (Human). This target record connects NCBI Gene 7039, UniProtKB P01135 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7039
UniProtKB
P01135
ELK Biotechnology
£284.00