Rat ACP5(Acid Phosphatase 5, Tartrate Resistant) ELISA Kit from ELK Biotechnology
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Rat ACP5(Acid Phosphatase 5, Tartrate Resistant) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring ACP5 in Serum, Plasma, Tissue homogenates, Other biological fluids samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ACP5 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include TRAP; TrATPase; Tartrate Resistant Acid Phosphatase; Type 5 acid phosphatase; TRACP 5; tartrate-resistant acid phosphatase 5b.
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Sample compatibility evidence using the same validation key.
| Sample type | Serum | Plasma | Tissue homogenates | Other biological fluids |
|---|---|---|---|---|
| Sample compatibility |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
Acid phosphatase 5, tartrate resistant (ACP5) encodes tartrate-resistant acid phosphatase type 5 (TRAP/TR-AP), an iron-containing acid phosphatase. Human ACP5 maps to NCBI Gene 54 and reviewed UniProtKB P13686; this target record links the verified biology to related ABMIUM catalogue products.
Biological function: ACP5/TRAP catalyses phosphate-monoester hydrolysis and is distinguished from other acid phosphatases by resistance to L(+)-tartrate inhibition. Reviewed UniProt describes tartrate-resistant acid phosphatase type 5 in osteopontin and bone sialoprotein dephosphorylation, supporting its established connection to bone and osteoclast biology.
