Manufactured by:ELK Biotechnology
RUO

EasyStep Human NLRP3(NLR Family, Pyrin Domain Containing Protein 3) ELISA Kit

EasyStep Human NLRP3(NLR Family, Pyrin Domain Containing Protein 3) ELISA Kit from ELK Biotechnology

SKU:
ABM48590
Product type
ELISA Kits
TargetEasyStep Human NLRP3
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

EasyStep Human NLRP3(NLR Family, Pyrin Domain Containing Protein 3) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring EasyStep Human NLRP3 in Serum, Plasma samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NLRP3, and the Human NLRP3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NLRP3. After TMB substrate solution is added, only those wells that contain Human NLRP3 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NLRP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include AVP; AGTAVPRL; AII; AII/AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCU; MWS; NALP3; PYPAF1; Cryopyrin; Cold Induced Autoinflammatory Syndrome 1; Caterpiller-Like Receptor 1.1.

Key facts & specifications
Research areas
Signal transduction
Also known as
AVP; AGTAVPRL; AII; AII/AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCU; MWS; NALP3; PYPAF1; Cryopyrin; Cold Induced Autoinflammatory Syndrome 1; Caterpiller-Like Receptor 1.1
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
37 pg/mL
Detection range
0.16-10 ng/mL
Plate format
96-well pre-coated
Form / buffer
Pre-coated microplate kit
Quantity per pack
48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSerumPlasma
Sample compatibility
Identifiers & database references
Supplier SKU / catalogue number
ELK039ES
UniProt entry
D4A523
Storage, stability & shipping
Storage conditions
Store according to the conditions stated in the product manual.
Long-term storage
Store according to the conditions stated in the product manual.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£366.00