Rat CRH(Corticotropin Releasing Hormone) ELISA Kit

Rat CRH(Corticotropin Releasing Hormone) ELISA Kit - ABMIUM

Rat CRH(Corticotropin Releasing Hormone) ELISA Kit - ABMIUM
Manufactured by:Elabscience
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Rat CRH(Corticotropin Releasing Hormone) ELISA Kit

Rat CRH(Corticotropin Releasing Hormone) ELISA Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM12420-1
Product type
ELISA Kits
TargetCRH

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Rat CRH(Corticotropin Releasing Hormone) ELISA Kit is a research-use ELISA kit from Elabscience for measuring CRH. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Key facts & specifications
Research areas
Cancer, Neuroscience, Signal transduction
Also known as
Crh,CRF
Cross-reactivity
This kit recognizes Rat CRH in samples.No significant cross-reactivity or interference between Rat CRH and analogues was observed
Incubation temperature
2-8℃
Assay type
Traditional ELISA Kits
Detection method
Colorimetric method,ELISA,Competitive
Plate format
48-well microplate plates pre-coated with anti-CRH capture antibody
Technical note
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat CRH. During the reaction, Rat CRH in the sample or standard competes with a fixed amount of Rat CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat CRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat CRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Identifiers & database references
Supplier SKU / catalogue number
E-EL-R0270_48T
Gene symbol
CRH
Gene ID
81648
UniProt accession
P01143
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CRHcorticotropin releasing hormone

View target guide

CRH identifies corticotropin releasing hormone in Homo sapiens (Human). This target record connects NCBI Gene 1392, UniProtKB P06850 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1392
UniProtKB
P06850
Browse all CRH productsRead target context & evidence
Elabscience
£295.00£206.50