Jurkat cells were incubated and cultured in medium with (10 mM 2-DG (2-Deoxy-D-glucose)) or without 2-DG (Untreated control) for 60 min, washed and then incubated with 2-NBDG Glucose Uptake Assay Kit for 60 min, and flow cytometric detection of The glucose uptake of the cells was detected by flow cytometry. Incubation with 2-DG inhibited the glucose uptake of Jurkat cells.
Manufactured by:Elabscience
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2-NBDG Glucose Uptake Cell-Based Kit

2-NBDG Glucose Uptake Cell-Based Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM10806-1
Product type
Assay Kits

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

2-NBDG Glucose Uptake Cell-Based Kit from Elabscience is intended for research workflows. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Incubation temperature
This product can be stored for 1 year at -20°C
Assay type
Assay Kits
Detection method
Fluorometric method, Flow cytometry
Technical note
2-NBDG Reagent is a green fluorescently labeled deoxyglucose analog with the same transporters (GLUTs) and similar transport kinetics as D-glucose.It is allowed to be phosphorylated by hexokinase because of a modification at the C-2 position.When it enters the cell, it is phosphorylated by hexokinase at the C-6 position to produce 2-NBDG-6-phosphate (a fluorescent derivative). Thus the fluorescence intensity reflects the glucose uptake.
Identifiers & database references
Supplier SKU / catalogue number
E-CK-A441_200Assays
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Elabscience
£399.00£319.20