Manufactured by:ELK Biotechnology
RUO

RHEB rabbit pAb

RHEB rabbit pAb from ELK Biotechnology

SKU:
ABM109675
Product type
Primary Antibody
TargetRHEB
ApplicationsWB, IHC, ELISA
Species / reactivityHuman, Mouse, Rat, Predicted, Cow
Expression systemUbiquitous. Highest levels observed in skeletal and cardiac muscle.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

RHEB rabbit pAb is a research-use antibody from ELK Biotechnology for studying RHEB in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat, Predicted, Cow. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of RHEB Polyclonal Antibody at Human;Mouse;Rat;Predicted:Cow, It doesn't reacte with total protein.
Validation evidence captions Western Blot analysis of mouse-kidney mouse-brain cells using RHEB Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Key facts & specifications
Research areas
>>Phospholipase D signaling pathway, >>Autophagy - animal, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Cellular senescence, >>Thermogenesis, >>Insulin signaling pathway, >>Thyroid hormone signaling pathway, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Herpes simplex virus 1 infection, >>Choline metabolism in cancer
Also known as
Ras homolog enriched in brain
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen
Synthetic Peptide of RHEB
Observed band
20kD
Cellular localization
Endomembrane system ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytosol . Endoplasmic reticulum membrane ; Lipid-anchor ; Cytoplasmic side .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Predicted
Identifiers & database references
Supplier SKU / catalogue number
ES8475
UniProt entry
Q15382
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00