DGK-η rabbit pAb
Manufactured by:ELK Biotechnology
RUO

DGK-η rabbit pAb

DGK-η rabbit pAb from ELK Biotechnology

SKU:
ABM108375
Product type
Primary Antibody
TargetDGK-η
ApplicationsIHC, IF, ELISA
Species / reactivityHuman, Mouse
Expression system[Isoform 1]: Expressed only in testis, kidney and colon. ; [Isoform 2]: Ubiquitously expressed.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

DGK-η rabbit pAb is a research-use antibody from ELK Biotechnology for studying DGK-η in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

diacylglycerol kinase eta(DGKH) Homo sapiens This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014],

Additional antibody information

Specificity DGK-η Polyclonal Antibody detects endogenous levels of DGK-η protein.
Validation evidence captions Immunofluorescence analysis of HeLa cells, using DGKH Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using DGKH Antibody. The picture on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Glycerolipid metabolism, >>Glycerophospholipid metabolism, >>Metabolic pathways, >>Phosphatidylinositol signaling system, >>Phospholipase D signaling pathway, >>Choline metabolism in cancer
Also known as
DGKH; Diacylglycerol kinase eta; DAG kinase eta; Diglyceride kinase eta; DGK-eta
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human DGKH. AA range:771-820
Cellular localization
Cytoplasm . Cell membrane . Translocated from the cytoplasm to endosomes in response to stress stimuli (PubMed:12810723). Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association (PubMed:12810723). Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS (PubMed:19710016).
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
135kD
Concentration
1 mg/ml
Working concentration
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 771-820
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES4959
UniProt entry
Q86XP1
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00