Manufactured by:ELK Biotechnology
RUO

MRLC2 rabbit pAb

MRLC2 rabbit pAb from ELK Biotechnology

SKU:
ABM107267
Product type
Primary Antibody
TargetMyosin Light Chain 2
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemSmooth muscle tissues and in some, but not all, nonmuscle cells.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

MRLC2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Myosin Light Chain 2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity MRLC2 Polyclonal Antibody detects endogenous levels of MRLC2 protein.
Validation evidence captions Western Blot analysis of various cells using MRLC2 Polyclonal Antibody | Immunofluorescence analysis of HUVEC cells, using MRLC2 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MRLC2 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COLO205 cells, using MRLC2 Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Vascular smooth muscle contraction, >>Axon guidance, >>Focal adhesion, >>Tight junction, >>Leukocyte transendothelial migration, >>Regulation of actin cytoskeleton, >>Oxytocin signaling pathway, >>Shigellosis, >>Salmonella infection
Also known as
MYL9; MLC2; MRLC1; MYRL2; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2; smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRL
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human MRLC2. AA range:3-52
Observed band
18kD
Cellular localization
Cytoplasm, cytoskeleton . Cytoplasm, cell cortex . Colocalizes with F-actin, MYH9 and PIEZO1 at the actomyosin cortex in myoblasts. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 3-52
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES2823
UniProt entry
P24844/P19105
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00