β-Catenin rabbit pAb

β-Catenin rabbit pAb

β-Catenin rabbit pAb
Manufactured by:ELK Biotechnology
RUO

β-Catenin rabbit pAb

β-Catenin rabbit pAb from ELK Biotechnology

SKU:
ABM106888
Product type
Primary Antibody
TargetCatenin-β
ApplicationsWB
Species / reactivityMouse, Rat, Rabbit
Expression systemExpressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level) (PubMed:29367600).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

β-Catenin rabbit pAb is a research-use antibody from ELK Biotechnology for studying Catenin-β in WB workflows with listed reactivity for Mouse, Rat, Rabbit. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016],

Additional antibody information

Specificity The antibody detects endogenous β-Catenin protein.
Validation evidence captions Western blot analysis of 1) 3T3, 2) Rat Liver, diluted at 1:2,000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Key facts & specifications
Research areas
>>Rap1 signaling pathway, >>Wnt signaling pathway, >>Hippo signaling pathway, >>Focal adhesion, >>Adherens junction, >>Signaling pathways regulating pluripotency of stem cells, >>Leukocyte transendothelial migration, >>Melanogenesis, >>Thyroid hormone signaling pathway, >>Cushing syndrome, >>Alcoholic liver disease, >>Alzheimer disease, >>Pathways of neurodegeneration - multiple diseases, >>Bacterial invasion of epithelial cells, >>Salmonella infection, >>Hepatitis C, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Pathways in cancer, >>Proteoglycans in cancer, >>Colorectal cancer, >>Endometrial cancer, >>Prostate cancer, >>Thyroid cancer, >>Basal cell carcinoma, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer, >>Arrhythmogenic right ventricular cardiomyopathy, >>Fluid shear stress and atherosclerosis
Also known as
CTNNB1; CTNNB; OK/SW-cl.35; Catenin beta-1; Beta-catenin
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB: 1:2000-4000
Immunogen
Recombinant Protein of Catenin beta-1
Observed band
92kD
Cellular localization
Cytoplasm . Nucleus . Cytoplasm, cytoskeleton . Cell junction, adherens junction . Cell junction . Cell membrane . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cell junction, synapse . Cytoplasm, cytoskeleton, cilium basal body . Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity). Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of cent
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB: 1:2000-4000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Mouse
Rat
Rabbit
Identifiers & database references
Supplier SKU / catalogue number
ES20585
UniProt entry
P35222
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00