Manufactured by:ELK Biotechnology
RUO

AP2B1 rabbit pAb

AP2B1 rabbit pAb from ELK Biotechnology

SKU:
ABM106604
Product type
Primary Antibody
TargetAP2B1
ApplicationsWB
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed in the brain (at protein level).
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

AP2B1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying AP2B1 in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of AP2B1 at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Endocytosis, >>Synaptic vesicle cycle, >>Endocrine and other factor-regulated calcium reabsorption, >>Huntington disease
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000
Immunogen
Synthesized peptide derived from human AP2B1 AA range: 70-120
Cellular localization
Cell membrane . Membrane, coated pit ; Peripheral membrane protein ; Cytoplasmic side . AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
103kD
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 70-120
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES18309
UniProt entry
P63010
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00