mTOR (phospho Thr2446) rabbit pAb from ELK Biotechnology
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mTOR (phospho Thr2446) rabbit pAb is a research-use antibody from ELK Biotechnology for studying mTOR in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008],
| Specificity | Phospho-mTOR (T2446) Polyclonal Antibody detects endogenous levels of mTOR protein only when phosphorylated at T2446. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-mTOR (T2446) Polyclonal Antibody diluted at 1:1000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using mTOR (Phospho-Thr2446) Antibody | Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15', using mTOR (Phospho-Thr2446) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
Mechanistic target of rapamycin kinase (MTOR), commonly called mTOR, is a serine/threonine protein kinase and core component of mTOR signalling complexes. This ABMIUM record connects NCBI Gene 2475 and UniProtKB P42345 to catalogue-backed research products.
Biological function: Mechanistic target of rapamycin kinase (mTOR/MTOR) is the catalytic kinase of mTORC1 and mTORC2. These complexes integrate nutrient, energy and growth-factor signals to regulate processes including protein synthesis, cell growth, metabolism, survival and cytoskeletal organisation.
