Manufactured by:ELK Biotechnology
RUO

eIF4A1 Mouse mAb

eIF4A1 Mouse mAb from ELK Biotechnology

SKU:
ABM102338
Product type
Primary Antibody
TargeteIF4A1
ApplicationsWB, IHC, IF
Species / reactivityHuman, Rat, Mouse

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

eIF4A1 Mouse mAb is a research-use antibody from ELK Biotechnology for studying eIF4A1 in WB, IHC, IF workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA - this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression.

Additional antibody information

Validation evidence captions Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with EM1136 diluted at 1:2,000. | IF analysis of Hela with EM1136(Left) and DAPI (Right) diluted at 1:100. | Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using eIF4A1(EM1136)Mouse mAb diluted at 1:200.
Key facts & specifications
Research areas
Antibody Research
Also known as
DDX2, eIF 4A I, Eukaryotic initiation factor 4A-1, ATP-dependent RNA helicase eIF4A-1,DDX2A
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Dilution range
WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200
Immunogen
Synthetic Peptide
Observed band
48kDa
Cellular localization
Cytosol,Extracellular region or secreted
Assay type
Antibody validation/application
Source organism
Mouse
Concentration
1mg/mL
Working concentration
WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
EM1136
UniProt entry
P60842
Storage, stability & shipping
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage warning
Avoid repeated freeze-thaw cycles.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00