Primary Antibody supplied by Boster Bio
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Anti-Macrosialin CD68 Antibody Picoband® is a research-use antibody from Boster Bio for studying Macrosialin in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB workflows with listed reactivity for Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Superstar antibody: Boster’s anti mouse CD68 antibody (PA1518) is among its top 10 bestselling antibodies, and one of the most cited mouse/rat CD68 antibodies on the market. Because of this antibody’s high demand, we have subsequently developed a rabbit monoclonal anti CD68 antibody (M00602-1) targetting a similar epitope.
High specificity: PA1518 reacts with murine macrosialin/CD68, and has been validated with Western blotting and confirmed its stellar specificity. CD68 is a highly glycosylated protein, and its expected western blot molecular weight is between 80kDa to 110kDa, depending on glycosylation level. PA1518’s observed MW in WB is near 90-100kDa. Testing on negative control tissues showed no significant bands (images available on request) or staining. See more info in the positive and negative control design section. Based on immunogen sequence homology, this antibody is not expected to cross react with other proteins from the LAMP family, which complies with QC testing observations.
Great for CD68 IHC: This antibody produces clean and specific immunobiological stains in both mouse and rat tissues. Our QC team has validated it on spleen and liver tissues of both mice and rats. Click product images for more details on experiment conditions.
More about CD68: CD68 is a cell surface marker often used to identify macrophages and other cell types in the monocyte lineage. It is a transmembrane protein that binds to electins and selectins and plays a role in macrophage homing movement. Check out the CD68 biomarker page for more information on CD68 and view all CD68 antibodies, ELISA kits and proteins.
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.CD68 is a heavily glycosylated glycoprotein that is highly expressed in macrophages and other mononuclear phagocytes. Macrosialin, if not glycosylated, has the main sequence that consists of 354 amino acids with an expected molecular weight of 37.4 kD. CD68, which can be located in the cytoplasmic granules of a variety of blood cells and myocytes, can be detected via immunohistochemistry. It's particularly effective as a marker for monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts, all of which belong to the macrophage lineage. CD68 is expressed in tissue macrophages and to a lesser extent in dendritic cells. CD68 is involved in tissue macrophage phagocytic activities, as well as intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It works by binding to tissue and organ-specific lectins or selectins, enabling macrophage subsets to be ed to specific locations. Macrophages may be able to crawl over selectin-bearing substrates or other cells if CD68 is rapidly recirculated from endosomes and lysosomes to the plasma membrane.
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | PA1518 |
| Product category | Primary Antibodies |
| Reactive species | Mouse, Rat |
| Tested applications | ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB |
| Available conjugations | Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647 |
| Gene name | CD68 |
| Protein name | Macrosialin |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of mouse CD68, different from the related rat sequence by one amino acid. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Purification | Immunogen affinity purified. |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | P31996 |
| NCBI Gene ID | 12514 |
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
| Biotin | 100 μg/vial |
| Cy3 | 100 μg/vial |
| Fluoro488 | 100 μg/vial |
| Fluoro550 | 100 μg/vial |
| Fluoro594 | 100 μg/vial |
| FITC | 100 μg/vial |
| HRP | 100 μg/vial |
| APC | 100 μg/vial |
| PE | 100 μg/vial |
| Fluoro647 | 100 μg/vial |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | Flow Cytometry | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Mouse | ||||||||
| Rat |
This product is manufactured by Boster Bio and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
