Manufactured by:Boster Bio
ABMIUM Verified™RUO

Anti-CD146/MCAM Antibody Picoband® (monoclonal, 4C12

Primary Antibody supplied by Boster Bio

SKU:
ABM23013-1
Product type
Primary Antibodies
TargetCell surface glycoprotein MUC18
ApplicationsELISA, Flow Cytometry, IHC, IHC-F, IHC-P, WB
Species / reactivityHuman
PurityNo cross reactivity with other proteins.

Manufacturer provenance

Manufactured by:
Boster Bio
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Anti-CD146/MCAM Antibody Picoband® (monoclonal, 4C12 is a research-use antibody from Boster Bio for studying Cell surface glycoprotein MUC18 in ELISA, Flow Cytometry, IHC, IHC-F, IHC-P, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-CD146/MCAM Antibody Picoband® (monoclonal, 4C12) catalog # M01683-3. Tested in Flow Cytometry, IHC, IHC-F, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.

Product information

Key facts & specifications
Also known as
CD146; MCAM; MUC18
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: 4C12
Isotype
Mouse IgG2a
Cross-reactivity
No cross-reactivity with other proteins.
Dilution range
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Immunogen
E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.
Observed band
120 kDa
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification
Immunogen affinity purified.
Form / buffer
Lyophilized
Sequence fragment
E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Identifiers & database references
Supplier SKU / catalogue number
M01683-3
Gene symbol
MCAM
Gene ID
4162
UniProt accession
P43121
Storage, stability & shipping
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Storage warning
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Compliance & documents
CE marked
No
Datasheet
Open datasheet
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Boster Bio and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Boster Bio
£328.00