The mouse bone marrow-derived dendritic cells cultured in vitro were matured by LPS, and co-cultured withthe spleen of 4T1 tumor-bearing mice (E-CK-A345 staining). After co-staining with Elab Fluor<sup>®</sup> Red 780Anti-Mouse CD8a Antibody (E-AB-F1104S) and APC Anti-Mouse CD4 (E-AB-F1097E), detect theproliferation of CD8+T cells.
Manufactured by:Elabscience
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CFSE Cell Division Tracker Kit

CFSE Cell Division Tracker Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM10777-1
Product type
Assay Kits

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

CFSE Cell Division Tracker Kit from Elabscience is intended for research workflows. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Incubation temperature
This product can be stored at -20°C for 12 months with shading light.
Assay type
Assay Kits
Detection method
Fluorometric method
Technical note
CFDA SE is a kind of fluorescein dye with membrane permeability, which has no fluorescence itself. When CFDA SE enters living cells through the cell membrane, it can be catalyzed by esterase in cytoplasm to produce carboxyfluorescein succinimide ester (CFSE), which can emit strong green fluorescence, can not penetrate the cell membrane, and can remain intact in the cell. CFSE can also spontaneously and irreversibly combine with amino groups of intracellular proteins to couple to these proteins. At the same time, excessive and uncoupled CFDA SE is passively diffused back into extracellular medium and removed by subsequent washing steps.
Identifiers & database references
Supplier SKU / catalogue number
E-CK-A345_2000Assays
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Elabscience
£335.00£268.00