Manufactured by:ELK Biotechnology
RUO

Na+/K+-ATPase α1 (Phospho-Tyr260) rabbit pAb

Na+/K+-ATPase α1 (Phospho-Tyr260) rabbit pAb from ELK Biotechnology

SKU:
ABM109823
Product type
Primary Antibody
TargetNa+/K+-ATPase α1
ApplicationsWB, IHC
Species / reactivityHuman, Mouse, Rat
Expression systemBrain,Cerebellum,Cervix,Placenta,Retinal pigment epithelium
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Na+/K+-ATPase α1 (Phospho-Tyr260) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Na+/K+-ATPase α1 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

Additional antibody information

Specificity This antibody detects endogenous levels of Na+/K+-ATPase α1 (Phospho-Tyr260).
Validation evidence captions Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Key facts & specifications
Research areas
>>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Cardiac muscle contraction, >>Adrenergic signaling in cardiomyocytes, >>Insulin secretion, >>Thyroid hormone synthesis, >>Thyroid hormone signaling pathway, >>Aldosterone synthesis and secretion, >>Aldosterone-regulated sodium reabsorption, >>Endocrine and other factor-regulated calcium reabsorption, >>Proximal tubule bicarbonate reclamation, >>Salivary secretion, >>Gastric acid secretion, >>Pancreatic secretion, >>Carbohydrate digestion and absorption, >>Protein digestion and absorption, >>Bile secretion, >>Mineral absorption
Also known as
Sodium/potassium-transporting ATPase subunit alpha-1 (Na(+)/K(+) ATPase alpha-1 subunit) (EC 3.6.3.9) (Sodium pump subunit alpha-1)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Immunogen
Synthesized phospho derived from human Na+/K+-ATPase α1 (Phospho-Tyr260) Polyclonal
Observed band
115kD
Cellular localization
Basolateral cell membrane ; Multi-pass membrane protein . Cell membrane, sarcolemma ; Multi-pass membrane protein . Cell projection, axon . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES8885
UniProt entry
P05023
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00