Manufactured by:ELK Biotechnology
RUO

TRIF rabbit pAb

TRIF rabbit pAb from ELK Biotechnology

SKU:
ABM109689
Product type
Primary Antibody
TargetTRIF
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemUbiquitously expressed but with higher levels in liver.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

TRIF rabbit pAb is a research-use antibody from ELK Biotechnology for studying TRIF in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response. [provided by RefSeq, Jan 2012],

Additional antibody information

Specificity TRIF Polyclonal Antibody detects endogenous levels of TRIF
Validation evidence captions Western Blot analysis of HEPG2 cells using TRIF Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200
Key facts & specifications
Research areas
>>NF-kappa B signaling pathway, >>Necroptosis, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>Alcoholic liver disease, >>Pertussis, >>Yersinia infection, >>Chagas disease, >>Hepatitis C, >>Hepatitis B, >>Influenza A, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Herpes simplex virus 1 infection, >>PD-L1 expression and PD-1 checkpoint pathway in cancer, >>Lipid and atherosclerosis
Also known as
TIR domain-containing adapter molecule 1 (TICAM-1;Proline-rich, vinculin and TIR domain-containing protein B;Putative NF-kappa-B-activating protein 502H;Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta;TIR domain-containing adapter protein inducing IFN-beta)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000,IHC-p 1:50-200 ELISA 1:10000-20000
Immunogen
Synthesized peptide derived from TRIF at AA range: 663-712
Observed band
80kD
Cellular localization
Cytoplasmic vesicle, autophagosome . Cytoplasm, cytosol . Mitochondrion . Colocalizes with UBQLN1 in the autophagosome (PubMed:21695056). Colocalizes in the cytosol with DDX1, DDX21 and DHX36. Colocalizes in the mitochondria with DDX1 and poly(I:C) RNA ligand. The multi-helicase-TICAM1 complex may translocate to the mitochondria upon poly(I:C) RNA ligand stimulation (By similarity). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000,IHC-p 1:50-200 ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 663-712
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES8501
UniProt entry
Q8IUC6
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00