Manufactured by:ELK Biotechnology
RUO

RIP140 (Acetyl Lys158) rabbit pAb

RIP140 (Acetyl Lys158) rabbit pAb from ELK Biotechnology

SKU:
ABM109655
Product type
Primary Antibody
TargetRIP140
ApplicationsWB, IHC, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemBrain,Epithelium,Mammary gland,Skin,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

RIP140 (Acetyl Lys158) rabbit pAb is a research-use antibody from ELK Biotechnology for studying RIP140 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Nuclear receptor interacting protein 1 (NRIP1) is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein modulates transcriptional activity of the estrogen receptor. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Acetyl-RIP140 (K158) Polyclonal Antibody detects endogenous levels of RIP140 around the acetylation site of K158 protein.
Validation evidence captions Western blot analysis of 293T using Acetyl-RIP140 (K158) antibody. Antibody was diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Key facts & specifications
Research areas
Antibody Research
Also known as
NRIP1; Nuclear receptor-interacting protein 1; Nuclear factor RIP140; Receptor-interacting protein 140
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen
Synthesized acetyl-peptide derived from human RIP140 around the acetylation site of K158.
Cellular localization
Nucleus . Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES8434
UniProt entry
P48552
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00