Manufactured by:ELK Biotechnology
RUO

Vav2 (phospho Tyr142) rabbit pAb

Vav2 (phospho Tyr142) rabbit pAb from ELK Biotechnology

SKU:
ABM109350
Product type
Primary Antibody
TargetVAV2
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse
Expression systemWidely expressed.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Vav2 (phospho Tyr142) rabbit pAb is a research-use antibody from ELK Biotechnology for studying VAV2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],

Additional antibody information

Specificity Phospho-Vav2 (Y142) Polyclonal Antibody detects endogenous levels of Vav2 protein only when phosphorylated at Y142.
Validation evidence captions Immunofluorescence analysis of NIH/3T3 cells, using VAV2 (Phospho-Tyr142) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using VAV2 (Phospho-Tyr142) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HeLa cells and K562 cells treated with TNF 20ng/ml 30', using VAV2 (Phospho-Tyr142) Antibody. The lane on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Rap1 signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>Focal adhesion, >>Natural killer cell mediated cytotoxicity, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>Fc epsilon RI signaling pathway, >>Fc gamma R-mediated phagocytosis, >>Leukocyte transendothelial migration, >>Regulation of actin cytoskeleton, >>Yersinia infection, >>Proteoglycans in cancer, >>Lipid and atherosclerosis
Also known as
VAV2; Guanine nucleotide exchange factor VAV2; VAV-2
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human VAV2 around the phosphorylation site of Tyr142. AA range:108-157
Observed band
101kD
Cellular localization
cytosol,plasma membrane,
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 108-157
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES7489
UniProt entry
P52735
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00