Sox-9 (phospho Ser181) rabbit pAb from ELK Biotechnology
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Sox-9 (phospho Ser181) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Sox-9 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
SRY-box 9(SOX9) Homo sapiens The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-Sox-9 (S181) Polyclonal Antibody detects endogenous levels of Sox-9 protein only when phosphorylated at S181. |
|---|---|
| Validation evidence captions | Western blot analysis of Sox-9 (phospho Ser181) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with UV, 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour. | Immunofluorescence analysis of A549 cells, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from 293 cells treated with PBS 60', using SOX-9 (Phospho-Ser181) Antibody. The lane on the right is blocked with the phospho peptide. |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
