MEK-1 (phospho Thr386) rabbit pAb

MEK-1 (phospho Thr386) rabbit pAb

MEK-1 (phospho Thr386) rabbit pAb
Manufactured by:ELK Biotechnology
RUO

MEK-1 (phospho Thr386) rabbit pAb

MEK-1 (phospho Thr386) rabbit pAb from ELK Biotechnology

SKU:
ABM109116
Product type
Primary Antibody
TargetMEK-1
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemWidely expressed, with extremely low levels in brain.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

MEK-1 (phospho Thr386) rabbit pAb is a research-use antibody from ELK Biotechnology for studying MEK-1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-MEK-1 (T386) Polyclonal Antibody detects endogenous levels of MEK-1 protein only when phosphorylated at T386.
Validation evidence captions Western Blot analysis of 3T3-EGF cells using Phospho-MEK-1 (T386) Polyclonal Antibody diluted at 1:2000 | Western blot analysis of MAP2K1 (Phospho-Thr386) Antibody. The lane on the right is blocked with the MAP2K1 (Phospho-Thr386) peptide.
Key facts & specifications
Research areas
>>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>MAPK signaling pathway, >>ErbB signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>HIF-1 signaling pathway, >>FoxO signaling pathway, >>Sphingolipid signaling pathway, >>Phospholipase D signaling pathway, >>Oocyte meiosis, >>Autophagy - animal, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>Apoptosis, >>Cellular senescence, >>Vascular smooth muscle contraction, >>VEGF signaling pathway, >>Apelin signaling pathway, >>Osteoclast differentiation, >>Focal adhesion, >>Gap junction, >>Signaling pathways regulating pluripotency of stem cells, >>Neutrophil extracellular trap formation, >>Toll-like receptor signaling pathway, >>Natural killer cell mediated cytotoxicity, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>Fc epsilon RI signaling pathway, >>Fc gamma R-mediated phagocytosis, >>TNF signaling pathway, >>Lon
Also known as
MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human MAP2K1 around the phosphorylation site of Thr386. AA range:344-393
Observed band
45kD
Cellular localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body . Cytoplasm . Nucleus . Membrane ; Peripheral membrane protein . Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis (PubMed:14737111). Membrane localization is probably regulated by its interaction with KSR1 (PubMed:10409742). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 344-393
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES6822
UniProt entry
Q02750
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00