Manufactured by:ELK Biotechnology
RUO

ZDHHC2 rabbit pAb

ZDHHC2 rabbit pAb from ELK Biotechnology

SKU:
ABM109005
Product type
Primary Antibody
TargetZDHHC2
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat, Monkey
Expression systemUbiquitously expressed (PubMed:10918388, PubMed:22034844). Reduced expression in colorectal cancers with liver metastasis (PubMed:10918388).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

ZDHHC2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ZDHHC2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

catalytic activity:Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.,disease:Mutations in ZDHHC2 are found in hepatocellular carcinoma and colorectal cancer.,domain:The DHHC domain is required for palmitoyltransferase activity.,function:Palmitoyltransferase specific for GAP43 and DLG4/PSD95.,similarity:Belongs to the DHHC palmitoyltransferase family.,similarity:Contains 1 DHHC-type zinc finger.,tissue specificity:Ubiquitously expressed. Reduced expression in colorectal cancers.,

Additional antibody information

Specificity ZDHHC2 Polyclonal Antibody detects endogenous levels of ZDHHC2 protein.
Validation evidence captions Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
Antibody Research
Also known as
ZDHHC2; REAM; REC; ZNF372; Palmitoyltransferase ZDHHC2; Reduced expression associated with metastasis protein; Ream; Reduced expression in cancer protein; Rec; Zinc finger DHHC domain-containing protein 2; DHHC-2; Zinc finger protein 372
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human ZDHHC2. AA range:61-110
Observed band
42kD
Cellular localization
Cell junction, synapse, postsynaptic density . Postsynaptic recycling endosome membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Translocates to postsynaptic density when synaptic activity decreases. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 61-110
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Monkey
Identifiers & database references
Supplier SKU / catalogue number
ES6503
UniProt entry
Q9UIJ5
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00