Manufactured by:ELK Biotechnology
RUO

NMBR rabbit pAb

NMBR rabbit pAb from ELK Biotechnology

SKU:
ABM108963
Product type
Primary Antibody
TargetNMBR
ApplicationsWB, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed in epididymis (at protein level).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

NMBR rabbit pAb is a research-use antibody from ELK Biotechnology for studying NMBR in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a 7-transmembrane G protein-coupled receptor that binds neuromedin B, which is a growth factor and mitogen for gastrointestinal epithelial tissue and for normal and neoplastic lung. This receptor may play a role in smooth muscle contraction, neuronal responses, and the regulation of cell growth. Antagonists of this receptor have a potential therapeutic use in inhibiting tumor cell growth. Polymorphisms in this gene may be associated with a susceptibility for schizophrenia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2016],

Additional antibody information

Specificity NMBR Polyclonal Antibody detects endogenous levels of NMBR protein.
Validation evidence captions Western Blot analysis of 293 cells using NMBR Polyclonal Antibody | Immunofluorescence analysis of A549 cells, using NMBR Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from 293 and Jurkat cells, using NMBR Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of 1) Human Brain Tissue, 2) Rat Brain Tissue using NMBR Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Key facts & specifications
Research areas
>>Neuroactive ligand-receptor interaction
Also known as
NMBR; Neuromedin-B receptor; NMB-R; Epididymis tissue protein Li 185a; Neuromedin-B-preferring bombesin receptor
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human NMBR. AA range:221-270
Observed band
43kD
Cellular localization
Cell membrane ; Multi-pass membrane protein .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 221-270
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES6395
UniProt entry
P28336
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00