Manufactured by:ELK Biotechnology
RUO

GFAP (phospho Ser38) rabbit pAb

GFAP (phospho Ser38) rabbit pAb from ELK Biotechnology

SKU:
ABM108607
Product type
Primary Antibody
TargetGFAP
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemExpressed in cells lacking fibronectin.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

GFAP (phospho Ser38) rabbit pAb is a research-use antibody from ELK Biotechnology for studying GFAP in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008],

Additional antibody information

Specificity Phospho-GFAP (S38) Polyclonal Antibody detects endogenous levels of GFAP protein only when phosphorylated at S38.
Validation evidence captions Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GFAP (Phospho-Ser38) Antibody | Immunofluorescence analysis of COS7 cells, using GFAP (Phospho-Ser38) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using GFAP (Phospho-Ser38) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HeLa cells, using GFAP (Phospho-Ser38) Antibody. The lane on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>JAK-STAT signaling pathway
Also known as
GFAP; Glial fibrillary acidic protein; GFAP
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human GFAP around the phosphorylation site of Ser38. AA range:11-60
Observed band
50kD
Cellular localization
Cytoplasm . Associated with intermediate filaments. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 11-60
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES5509
UniProt entry
P14136
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

GFAPglial fibrillary acidic protein

View target guide

GFAP identifies glial fibrillary acidic protein in Homo sapiens (Human). This target record connects NCBI Gene 2670, UniProtKB P14136 and related ABMIUM catalogue products.

Biological function: GFAP is a class III intermediate-filament protein expressed prominently in astrocytes and other glial populations. It contributes to cytoskeletal organisation, maintenance of cell structure and glial responses to injury or stress.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2670
UniProtKB
P14136
ELK Biotechnology
£220.00