Manufactured by:ELK Biotechnology
RUO

DAPK2 (phospho Ser318) rabbit pAb

DAPK2 (phospho Ser318) rabbit pAb from ELK Biotechnology

SKU:
ABM108551
Product type
Primary Antibody
TargetDAPK2
ApplicationsIHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

DAPK2 (phospho Ser318) rabbit pAb is a research-use antibody from ELK Biotechnology for studying DAPK2 in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-DAPK2 (S318) Polyclonal Antibody detects endogenous levels of DAPK2 protein only when phosphorylated at S318.
Validation evidence captions Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DAPK2 (Phospho-Ser318) Antibody | Immunofluorescence analysis of COS7 cells, using DAPK2 (Phospho-Ser318) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using DAPK2 (Phospho-Ser318) Antibody. The picture on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Autophagy - animal, >>Pathways in cancer, >>Bladder cancer
Also known as
DAPK2; Death-associated protein kinase 2; DAP kinase 2; DAP-kinase-related protein 1; DRP-1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human DAPK2 around the phosphorylation site of Ser318. AA range:284-333
Cellular localization
Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
43kD
Concentration
1 mg/ml
Working concentration
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 284-333
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES5370
UniProt entry
Q9UIK4
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00