Manufactured by:ELK Biotechnology
RUO

EphA4 (phospho Tyr596) rabbit pAb

EphA4 (phospho Tyr596) rabbit pAb from ELK Biotechnology

SKU:
ABM108449
Product type
Primary Antibody
TargetEphA4
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse
Expression systemUbiquitous.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

EphA4 (phospho Tyr596) rabbit pAb is a research-use antibody from ELK Biotechnology for studying EphA4 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015],

Additional antibody information

Specificity Phospho-EphA4 (Y596) Polyclonal Antibody detects endogenous levels of EphA4 protein only when phosphorylated at Y596.
Validation evidence captions Western Blot analysis of JK cells using Phospho-EphA4 (Y596) Polyclonal Antibody
Key facts & specifications
Research areas
>>Axon guidance
Also known as
EPHA4; HEK8; SEK; TYRO1; Ephrin type-A receptor 4; EPH-like kinase 8; EK8; hEK8; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEK
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human EphA4 (phospho Tyr596)
Observed band
110kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Cell projection, axon . Cell projection, dendrite . Cell junction, synapse, postsynaptic density membrane . Early endosome . Cell junction, adherens junction . Clustered upon activation and targeted to early endosome. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES5128
UniProt entry
P54764
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

EPHA4EPH receptor A4

View target guide

EPHA4 identifies EPH receptor A4 in Homo sapiens (Human). This target record connects NCBI Gene 2043, UniProtKB P54764 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2043
UniProtKB
P54764
ELK Biotechnology
£220.00