Manufactured by:ELK Biotechnology
RUO

Cathepsin G rabbit pAb

Cathepsin G rabbit pAb from ELK Biotechnology

SKU:
ABM108352
Product type
Primary Antibody
TargetCathepsin G
ApplicationsWB, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemExpressed in neutrophils (at protein level) (PubMed:3799965). Expressed in B cells (PubMed:15100291).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Cathepsin G rabbit pAb is a research-use antibody from ELK Biotechnology for studying Cathepsin G in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Sep 2014],

Additional antibody information

Specificity Cathepsin G Polyclonal Antibody detects endogenous levels of Cathepsin G protein.
Validation evidence captions Western Blot analysis of COLO205 cells using Cathepsin G Polyclonal Antibody | Western blot analysis of lysate from COLO cells, using Cathepsin?G antibody.
Key facts & specifications
Research areas
>>Neuroactive ligand-receptor interaction, >>Lysosome, >>Neutrophil extracellular trap formation, >>Renin-angiotensin system, >>Amoebiasis, >>Systemic lupus erythematosus
Also known as
CTSG; Cathepsin G; CG
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Cathepsin G. AA range:67-116
Observed band
22kD
Cellular localization
Cell membrane ; Peripheral membrane protein . Cytoplasmic granule . Secreted . Cytoplasm, cytosol . Lysosome . Nucleus . Secreted by activated neutrophils (PubMed:3390156). Detected in synovial fluid (PubMed:32144329). Localizes to lysosomes in B cells where it is not endogenously synthesized but is internalized from the cell membrane (PubMed:15100291). Localizes to the nucleus during apoptosis (PubMed:11259672). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 67-116
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES4900
UniProt entry
P08311
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00