Manufactured by:ELK Biotechnology
RUO

Histone H2A (Phospho Ser129) rabbit pAb

Histone H2A (Phospho Ser129) rabbit pAb from ELK Biotechnology

SKU:
ABM106885
Product type
Primary Antibody
TargetHistone H2A
ApplicationsWB
Species / reactivityHuman, Mouse, Rat
Expression systemBone,Brain,Colon,Eye,Lymph,PCR rescued clones,Placenta,Sple
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Histone H2A (Phospho Ser129) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Histone H2A in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Additional antibody information

Specificity The antibody detects endogenous Histone H2A (Phospho Ser129) protein.
Validation evidence captions Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Key facts & specifications
Research areas
>>Necroptosis, >>Neutrophil extracellular trap formation, >>Alcoholism, >>Systemic lupus erythematosus
Also known as
HIST1H2AG; H2AFP; HIST1H2AI; H2AFC; HIST1H2AK; H2AFD; HIST1H2AL; H2AFI; HIST1H2AM; H2AFN; Histone H2A type 1; H2A.1; Histone H2A/p; HIST2H2AA3; H2AFO; HIST2H2AA; HIST2H2AA4; Histone H2A type 2-A; Histone H2A.2; Histone H2A/o; HIST3H2A; Histone H2A type 3
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB: 1:1000-2000
Immunogen
Synthetic Peptide of Histone H2A (Phospho Ser129)
Observed band
14kD
Cellular localization
Nucleus. Chromosome.
Assay type
Antibody validation/application
Source organism
Rabbit
Working concentration
WB: 1:1000-2000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Form / buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES20581
UniProt entry
P0C0S8/Q6FI13/Q7L7L0
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00