Manufactured by:ELK Biotechnology
RUO

Calpain 2 (Phospho Ser50) rabbit pAb

Calpain 2 (Phospho Ser50) rabbit pAb from ELK Biotechnology

SKU:
ABM106818
Product type
Primary Antibody
TargetCalpain 2
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemUbiquitous.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Calpain 2 (Phospho Ser50) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Calpain 2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],

Additional antibody information

Specificity This antibody detects endogenous levels of Human,Mouse,Rat Calpain 2 (Phospho Ser50)
Validation evidence captions Western Blot analysis of 1 A549 cell 2 LPS 100ng/mL 30min treated ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
Key facts & specifications
Research areas
>>Protein processing in endoplasmic reticulum, >>Apoptosis, >>Necroptosis, >>Cellular senescence, >>Focal adhesion, >>Alzheimer disease, >>Pathways of neurodegeneration - multiple diseases, >>Shigellosis
Also known as
Calpain-2 catalytic subunit (EC 3.4.22.53;Calcium-activated neutral proteinase 2;CANP 2;Calpain M-type;Calpain large polypeptide L2;Calpain-2 large subunit;Millimolar-calpain;M-calpain)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:1000-2000 ELISA 1:5000-20000
Immunogen
Synthesized peptide derived from human Calpain 2 (Phospho Ser50)
Observed band
69kD
Cellular localization
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES20175
UniProt entry
P17655
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00