p70 S6 kinase α (phospho Thr229) rabbit pAb from ELK Biotechnology
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p70 S6 kinase α (phospho Thr229) rabbit pAb is a research-use antibody from ELK Biotechnology for studying p70 S6 kinase α in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Pig. Specifications and supporting documents below should be reviewed when planning the experiment.
ribosomal protein S6 kinase B1(RPS6KB1) Homo sapiens This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013],
| Specificity | Phospho-p70 S6 kinase α (T229) Polyclonal Antibody detects endogenous levels of p70 S6 kinase α protein only when phosphorylated at T229. |
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| Validation evidence captions | Immunofluorescence analysis of Hela cell. 1,p70 S6 kinase α (phospho Thr229) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Caspase-8 Monoclonal Antibody(2G12)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min). | Western Blot analysis of various cells using Phospho-p70 S6 kinase α (T229) Polyclonal Antibody diluted at 1:500 | Western Blot analysis of LOVO cells using Phospho-p70 S6 kinase α (T229) Polyclonal Antibody diluted at 1:500 | Immunohistochemistry analysis of paraffin-embedded human brain, using p70 S6 Kinase (Phospho-Thr229) Antibody. The picture on the right is blocked with the phospho peptide. |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Pig |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
