Manufactured by:ELK Biotechnology
RUO

VPS45 rabbit pAb

VPS45 rabbit pAb from ELK Biotechnology

SKU:
ABM103925
Product type
Primary Antibody
TargetVPS45
ApplicationsWB
Species / reactivityHuman, Mouse, Rat
Expression systemUbiquitous. Expression was highest in testis, heart and brain, intermediate in kidney, spleen, prostate, ovary, small intestine and thymus and low in lung, skeletal muscle, placenta, colon, pancreas, peripheral blood leukocytes and liver.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

VPS45 rabbit pAb is a research-use antibody from ELK Biotechnology for studying VPS45 in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013],

Additional antibody information

Specificity This antibody detects endogenous levels of VPS45 at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Endocytosis
Also known as
Vacuolar protein sorting-associated protein 45 (h-VPS45) (hlVps45)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000
Immunogen
Synthesized peptide derived from human VPS45 AA range: 470-520
Observed band
65kD
Cellular localization
Golgi apparatus membrane ; Peripheral membrane protein . Endosome membrane ; Peripheral membrane protein . Associated with Golgi/endosomal vesicles and the trans-Golgi network. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 470-520
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES12347
UniProt entry
Q9NRW7
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.188% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00