Manufactured by:ELK Biotechnology
RUO

α-E-Catenin (phospho-Ser652) rabbit pAb

α-E-Catenin (phospho-Ser652) rabbit pAb from ELK Biotechnology

SKU:
ABM103689
Product type
Primary Antibody
Targetα-E-Catenin
ApplicationsWB
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed ubiquitously in normal tissues.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

α-E-Catenin (phospho-Ser652) rabbit pAb is a research-use antibody from ELK Biotechnology for studying α-E-Catenin in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

catenin alpha 1(CTNNA1) Homo sapiens This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016],

Additional antibody information

Specificity This antibody detects endogenous levels of Human Mouse Rat α-E-Catenin (phospho-Ser652)
Validation evidence captions Western Blot analysis of 1 MCF-7 treated with LPS, 2 MCF7,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
Key facts & specifications
Research areas
>>Hippo signaling pathway, >>Adherens junction, >>Leukocyte transendothelial migration, >>Bacterial invasion of epithelial cells, >>Pathways in cancer, >>Endometrial cancer, >>Gastric cancer, >>Arrhythmogenic right ventricular cardiomyopathy
Also known as
Catenin alpha-1 (Alpha E-catenin) (Cadherin-associated protein) (Renal carcinoma antigen NY-REN-13)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:1000-2000
Immunogen
Synthesized phosho peptide around human α-E-Catenin (Ser652)
Observed band
100kD
Cellular localization
[Isoform 1]: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.; [Isoform 3]: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES12062
UniProt entry
P35221
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00