Manufactured by:ELK Biotechnology
RUO

GNAI1 rabbit pAb

GNAI1 rabbit pAb from ELK Biotechnology

SKU:
ABM103624
Product type
Primary Antibody
TargetGNAI1
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemAdipocyte,Brain,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

GNAI1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GNAI1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],

Additional antibody information

Specificity GNAI1 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Rap1 signaling pathway, >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>Sphingolipid signaling pathway, >>Adrenergic signaling in cardiomyocytes, >>Axon guidance, >>Apelin signaling pathway, >>Gap junction, >>Platelet activation, >>Leukocyte transendothelial migration, >>Circadian entrainment, >>Retrograde endocannabinoid signaling, >>Glutamatergic synapse, >>Cholinergic synapse, >>Serotonergic synapse, >>GABAergic synapse, >>Dopaminergic synapse, >>Long-term depression, >>Progesterone-mediated oocyte maturation, >>Estrogen signaling pathway, >>Melanogenesis, >>Oxytocin signaling pathway, >>Regulation of lipolysis in adipocytes, >>Renin secretion, >>Relaxin signaling pathway, >>Parathyroid hormone synthesis, secretion and action, >>Cushing syndrome, >>Growth hormone synthesis, >>Gastric acid secretion, >>Parkinson disease, >>Cocaine addiction, >>Morphine addiction, >>Alcoholism, >>Pertussis, >>Chagas disease, >>Toxoplasmosis, >>Human cytomegalovirus infectio
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
38kD
Cellular localization
Nucleus . Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cell cortex . Membrane ; Lipid-anchor . Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody (PubMed:17635935). Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES11882
UniProt entry
P63096
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00