Manufactured by:Boster Bio
ABMIUM Verified™RUO

Anti-TNF alpha Antibody Picoband®

Primary Antibody supplied by Boster Bio

SKU:
ABM27983-1
Product type
Primary Antibodies
TargetElongation factor 1-alpha
ApplicationsELISA, Flow Cytometry, IHC, IHC-P, WB
Species / reactivityRat
PurityNo cross reactivity with other proteins.

Manufacturer provenance

Manufactured by:
Boster Bio
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Anti-TNF alpha Antibody Picoband® is a research-use antibody from Boster Bio for studying Elongation factor 1-alpha in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-TNF alpha Antibody Picoband® catalog # PB9010. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

TNF alpha (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference PB9010
Product category Primary Antibodies
Reactive species Rat
Tested applications ELISA, Flow Cytometry, IHC, IHC-P, WB
Available conjugations Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name TNF
Protein name Elongation factor 1-alpha
Specificity No cross reactivity with other proteins.
Immunogen E.coli-derived rat TNF alpha recombinant protein (Position: D89-L235). Rat TNF alpha shares 95% amino acid (aa) sequence identity with mouse TNF alpha.
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Purification Immunogen affinity purified.
Storage Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
UniProt P16599
NCBI Gene ID 24835

Available variants

Conjugation Size
Unconjugated 100 μg/vial
Biotin 100 μg/vial
Cy3 100 μg/vial
Fluoro488 100 μg/vial
Fluoro550 100 μg/vial
Fluoro594 100 μg/vial
FITC 100 μg/vial
HRP 100 μg/vial
APC 100 μg/vial
PE 100 μg/vial
Fluoro647 100 μg/vial
Key facts & specifications
Also known as
Cachectin; DIF; TNF; TNF a; TNF alpha; TNFA; TNF-alpha; TNFSF2; TNFα; TNF-α; Tumor necrosis factor
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Cross-reactivity
No cross-reactivity with other proteins
Dilution range
Western blot, 0.1-0.5μg/ml, Rat
Immunogen
E.coli-derived rat TNF alpha recombinant protein (Position: D89-L235). Rat TNF alpha shares 95% amino acid (aa) sequence identity with mouse TNF alpha.
Observed band
26 kDa
Molecular weight
25806 MW
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification
Immunogen affinity purified.
Form / buffer
Lyophilized
Sequence fragment
E.coli-derived rat TNF alpha recombinant protein (Position: D89-L235). Rat TNF alpha shares 95% amino acid (aa) sequence identity with mouse TNF alpha.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Rat
Identifiers & database references
Supplier SKU / catalogue number
PB9010
Gene symbol
TNF
Gene ID
24835
UniProt accession
P16599
Storage, stability & shipping
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Storage warning
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Compliance & documents
CE marked
No
Datasheet
Open datasheet
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Boster Bio and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Boster Bio
£328.00