- Research areas
- B Cell Chemotaxis, Migration, and Adhesion, Cytokines and Receptors in Tfh Cell Differentiation and Function, gamma delta T Cells, Helper T (Th) Cells, IL-17 Family, LTi Cells, Macrophage Activation Markers, Microglia Activation Markers, Molecules Secreted by VSMC, Multiple Sclerosis and Myelin Related Molecules, Natural Killer T (NKT) Cells, NCR-ILC3, Neuroinflammatory Factors, Search Cytokine and Growth Factor Products, T Cell Cytokine Signaling, Th17 Cells
- Also known as
- IL17A,CTLA-8,CTLA8,IL-17,IL-17A,IL17,Interleukin-17A,Cytotoxic T-lymphocyte-associated antigen 8
- Cross-reactivity
- This kit recognizes Human IL-17A in samples.No significant cross-reactivity or interference between Human IL-17A and analogues was observed
- Incubation temperature
- 2-8℃
- Assay type
- Traditional ELISA Kits
- Detection method
- Colorimetric method,ELISA,Sandwich
- Plate format
- 48-well microplate plates pre-coated with anti-IL-17A capture antibody
- Technical note
- This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-17A. You can calculate the concentration of Human IL-17A in the samples by comparing the OD of the samples to the standard curve.
