- Research areas
- Signal transduction, Cell Biology
- Also known as
- 60B8AG,CAGB,Calgranulin-B,Calprotectin L1H subunit,CFAG,CGLB,L1AG,Leukocyte L1 complex heavy chain,LIAG,MAC,MAC387,MIF,Migration inhibitory factor-related protein,Migration inhibitory factor-related protein 14,MRP,MRP14,MRP-14,NIF,P14,Protein S100-A,Protein S100-A9,S100 calcium-binding protein A,S100 calcium-binding protein A9,S100A,S100A9,S100B
- Cross-reactivity
- This kit recognizes Human CALP in samples. No significant cross-reactivity or interference between Human CALP and analogues was observed
- Incubation temperature
- 2-8℃
- Assay type
- Traditional ELISA Kits
- Detection method
- Colorimetric method,ELISA,Sandwich
- Plate format
- 48-well microplate plates pre-coated with anti-CALP capture antibody
- Technical note
- This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CALP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CALP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CALP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CALP. You can calculate the concentration of Human CALP in the samples by comparing the OD of the samples to the standard curve.
