Human SPARC ELISA Kit PicoKine®
ELISA Kit supplied by Boster Bio Read more ›
Product Description
SPARC (secreted protein acidic and rich in cysteine), also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | EK1210 |
| Product category | PicoKine® ELISA Kits |
| Reactive species | Human |
| Tested applications | ELISA |
| Gene name | SPARC |
| Protein name | SPARC |
| Sample type | cell culture supernatants, serum and human milk. |
| Assay range | 0.78 ng/ml - 50 ng/ml |
| Sensitivity | <20 pg/ml |
| Specificity | Natural and recombinant human SPARC |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: A18-I303 |
| Storage | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| UniProt | P09486 |
| NCBI Gene ID | 6678 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 96 wells/kit, with removable strips. |
Key Facts
| Target | SPARC |
| Also known as | Basement membrane protein 40; BM 40; ON; Osteonectin; SPARC |
| Species reactivity | Human |
| Purity | Natural and recombinant human SPARC |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Sensitivity | <20 pg/ml |
| Detection range | 0.78 ng/ml - 50 ng/ml |
| Sample types |
cell culture supernatantsserum and human milk.
See sample validation ›
|
Sample Type Validation
2 sample types| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants | |||||
| serum and human milk. |
Specifications
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.