Human OPN / Osteopontin ELISA Kit PicoKine®

Human OPN / Osteopontin ELISA Kit PicoKine® (96 Tests). Quantitate Human SPP1 in cell culture supernatants, serum, plasma (heparin, EDTA) , human milk and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EK0482
Product category	PicoKine® ELISA Kits
Reactive species	Human
Tested applications	ELISA
Gene name	SPP1
Protein name	Osteopontin
Sample type	cell culture supernatants, serum, plasma (heparin, EDTA) , human milk and urine.
Assay range	156 pg/ml - 10,000 pg/ml
Sensitivity	<10 pg/ml
Specificity	Natural and recombinant human OPN
Immunogen	Expression system for standard: NS0; Immunogen sequence: I17-N300
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	P10451
NCBI Gene ID	6696

Available variants

Conjugation	Size
Not applicable	96 wells/kit, with removable strips.