ELISA Kit ABMIUM Verified™Collaborator Tested
ABM-32825

Human CD13/Aminopeptidase N ELISA Kit PicoKine®

ABMIUM Verified™|Collaborator Tested|Boster Bio

ELISA Kit supplied by Boster Bio Read more ›

Related formats
Human CD13/Aminopeptidase N ELISA Kit PicoKine® Boster Bio
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Human CD13/Aminopeptidase N ELISA Kit PicoKine® Boster Bio

Product Description

Human CD13/Aminopeptidase N ELISA Kit PicoKine® (96 Tests). Quantitate Human ANPEP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference EK1216
Product category PicoKine® ELISA Kits
Reactive species Human
Tested applications ELISA
Gene name ANPEP
Protein name Aminopeptidase N
Sample type cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay range 312 pg/ml - 20,000 pg/ml
Sensitivity <10 pg/ml
Specificity Natural and recombinant human CD13
Immunogen Expression system for standard: NS0; Immunogen sequence: K69-K967
Storage Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt P15144
NCBI Gene ID 290

Available variants

Conjugation Size
Not applicable 96 wells/kit, with removable strips.

Key Facts

Product type ELISA Kit Target: Aminopeptidase N
Target Aminopeptidase N
Also known as Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; ANPEP; AP M; AP N; APN; CD13; gp150; hAPN; LAP1; Microsomal aminopeptidase; p150; PEPN
Species reactivity Human
Purity Natural and recombinant human CD13
Shelf life Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Sensitivity <10 pg/ml
Detection range 312 pg/ml - 20,000 pg/ml
Sample types
cell culture supernatantscell lysatesserum and plasma (heparinEDTA).
See sample validation ›
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Sample Type Validation

4 sample types
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
cell culture supernatants
cell lysates
serum and plasma (heparin
EDTA).

Specifications

Target
Aminopeptidase N
Also known as
Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; ANPEP; AP M; AP N; APN; CD13; gp150; hAPN; LAP1; Microsomal aminopeptidase; p150; PEPN
Purity
Natural and recombinant human CD13
Sensitivity
<10 pg/ml
Detection range
312 pg/ml - 20,000 pg/ml
Sample types
cell culture supernatantscell lysatesserum and plasma (heparinEDTA).
Species reactivity
Human

Storage & Stability

Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Long-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Storage conditions
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Shelf life
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Compliance & Certifications

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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