Tropomyosin α rabbit pAb
Tropomyosin α rabbit pAb from ELK Biotechnology Read more ›
Product Description
Tropomyosin α rabbit pAb is an ELK Biotechnology antibody for research workflows involving Tropomyosin α.
Background
This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by
Additional antibody information
| Specificity | Tropomyosin α Polyclonal Antibody detects endogenous levels of Tropomyosin α |
|---|---|
| Validation evidence captions | Western Blot analysis of 1,mouse-lung 2,mouse-brain 3,mouse-spleen cells using primary antibody diluted at 1:500(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour) |
Key Facts
| Target | Tropomyosin α |
| Also known as | Tropomyosin alpha-1 chain (Alpha-tropomyosin) (Tropomyosin-1) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000, ELISA 1:10000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 38kD |
| Cellular localization | Cytoplasm, cytoskeleton . Associates with F-actin stress fibers. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cardiac muscle contraction;>>Adrenergic signaling in cardiomyocytes;>>MicroRNAs in cancer;>>Hypertrophic cardiomyopathy;>>Dilated cardiomyopathy |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:500-2000, ELISA 1:10000-20000 |
| Immunogen | Synthesized peptide derived from human Tropomyosin α. at AA range: 101-150 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.