HNF-4α/γ (Acetyl Lys127/79) rabbit pAb
HNF-4α/γ (Acetyl Lys127/79) rabbit pAb from ELK Biotechnology Read more ›
Product Description
HNF-4α/γ (Acetyl Lys127/79) rabbit pAb is an ELK Biotechnology antibody for research workflows involving HNF-4α/γ.
Background
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012],
Additional antibody information
| Specificity | This antibody detects endogenous levels of HNF-4α/γ at Human:K127/79;Mouse:K127/79;Rat:K127, It doesn't reacte with total protein. |
|---|---|
| Validation evidence captions | Western blot analysis of mouse-lung mouse-brain mouse-heart Hela mouse-liver lysate, antibody was diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | HNF-4α/γ |
| Also known as | Hepatocyte nuclear factor 4-alpha (HNF-4-alpha) (Nuclear receptor subfamily 2 group A member 1) (Transcription factor 14) Hepatocyte nuclear factor 4-gamma (HNF-4-gamma) (Nuclear receptor subfamily 2 group A member 2)(TCF-14) (Transcription factor HNF-4) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000, ELISA 1:10000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 55kD |
| Cellular localization | Nucleus. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>AMPK signaling pathway;>>Maturity onset diabetes of the young |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:500-2000, ELISA 1:10000-20000 |
| Immunogen | Synthetic Acetyl peptide from human protein at AA range: 127(HNF-4α)/79(HNF-4γ) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.