ABM99829
Nanos Homologue 1 (NANOS1) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Nanos Homologue 1 (NANOS1) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Nanos Homologue 1 (NANOS1) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Nanos Homologue 1 (NANOS1).
Background
This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. [provided by RefSeq, Sep 2015],
Additional antibody information
| Specificity | Nanos Homologue 1 (NANOS1) Polyclonal Antibody detects endogenous levels of Nanos Homologue 1 (NANOS1) |
|---|---|
| Validation evidence captions | Western Blot analysis of 3T3 cells using Nanos Homologue 1 (NANOS1) Polyclonal Antibody diluted at 1:1500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200 |
Key Facts
Product type
Primary Antibody›
Target: Nanos Homologue 1 (NANOS1)
| Target | Nanos Homologue 1 (NANOS1) |
| Also known as | Nanos homolog 1 (NOS-1) (EC_Rep1a) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 30kD |
| Cellular localization | Cytoplasm, perinuclear region . Cytoplasm . Colocalizes with SNAPIN and PUM2 in the perinuclear region of germ cells. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCELISA |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen | Synthesized peptide derived from Nanos Homologue 1 (NANOS1) at AA range: 151-200 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Nanos Homologue 1 (NANOS1)
Also known as
Nanos homolog 1 (NOS-1) (EC_Rep1a)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCELISA
Immunogen
Synthesized peptide derived from Nanos Homologue 1 (NANOS1) at AA range: 151-200
Observed band
30kD
Cellular localization
Cytoplasm, perinuclear region . Cytoplasm . Colocalizes with SNAPIN and PUM2 in the perinuclear region of germ cells. .
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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