β-1,3-Gal-T2 rabbit pAb
β-1,3-Gal-T2 rabbit pAb from ELK Biotechnology Read more ›
Product Description
β-1,3-Gal-T2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving β-1,3-Gal-T2.
Background
This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3
Additional antibody information
| Specificity | β-1,3-Gal-T2 Polyclonal Antibody detects endogenous levels of β-1,3-Gal-T2 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using B3GALT2 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human heart tissue, using B3GALT2 Antibody. The picture on the right is blocked with the synthesized peptide. |
Key Facts
| Target | β-1,3-Gal-T2 |
| Also known as | B3GALT2; Beta-1; 3-galactosyltransferase 2; Beta-1,3-GalTase 2; Beta3Gal-T2; Beta3GalT2; UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase 2 |
| Concentration | 1 mg/ml |
| Working concentration | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Cellular localization | Golgi apparatus membrane ; Single-pass type II membrane protein . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Glycosphingolipid biosynthesis - lacto and neolacto series;>>Metabolic pathways |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IHCIFELISA |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human B3GALT2. AA range:373-422 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.