ABM99504
14-3-3 ζ/δ rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
14-3-3 ζ/δ rabbit pAb from ELK Biotechnology Read more ›
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Product Description
14-3-3 ζ/δ rabbit pAb is an ELK Biotechnology antibody for research workflows involving 14-3-3 ζ/δ.
Background
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],
Additional antibody information
| Specificity | 14-3-3 ζ/δ Polyclonal Antibody detects endogenous levels of 14-3-3 ζ/δ protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of NIH/3T3 cells, using 14-3-3 zeta/delta Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using 14-3-3 zeta/delta Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from K562 cells, using 14-3-3 zeta/delta Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: 14-3-3 ζ/δ
| Target | 14-3-3 ζ/δ |
| Also known as | YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 28kD |
| Cellular localization | Cytoplasm . Melanosome . Located to stage I to stage IV melanosomes. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cell cycle;>>Oocyte meiosis;>>PI3K-Akt signaling pathway;>>Hippo signaling pathway;>>Hepatitis C;>>Hepatitis B;>>Viral carcinogenesis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta/delta. AA range:196-245 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
14-3-3 ζ/δ
Also known as
YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta/delta. AA range:196-245
Observed band
28kD
Cellular localization
Cytoplasm . Melanosome . Located to stage I to stage IV melanosomes.
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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