PKC α (phospho Tyr658) rabbit pAb
PKC α (phospho Tyr658) rabbit pAb from ELK Biotechnology Read more ›
Product Description
PKC α (phospho Tyr658) rabbit pAb is an ELK Biotechnology antibody for research workflows involving PKC α.
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2
Additional antibody information
| Specificity | Phospho-PKC α (Y657) Polyclonal Antibody detects endogenous levels of PKC α protein only when phosphorylated at Y657. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293 cells using Phospho-PKC α (Y657) Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from COLO205 cells, using PKC alpha (Phospho-Tyr657) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
| Target | PKC α |
| Also known as | PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 80kD |
| Cellular localization | Cytoplasm . Cell membrane ; Peripheral membrane protein . Mitochondrion membrane ; Peripheral membrane protein . Nucleus . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>EGFR tyrosine kinase inhibitor resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Calcium signaling pathway;>>HIF-1 signaling pathway;>>Phosphatidylinositol signaling system;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Adrenergic signaling in cardiomyocytes;>>Vascular smooth muscle contraction;>>Wnt signaling pathway;>>Axon guidance;>>VEGF signaling pathway;>>Focal adhesion;>>Gap junction;>>Neutrophil extracellular trap formation;>>Natural killer cell mediated cytotoxicity;>>Fc epsilon RI signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Leukocyte transendothelial migration;>>Circadian entrainment;>>Long-term potentiation;>>Retrograde endocannabinoid signaling;>>Glutamatergic synapse;>>Cholinergic synapse;>>Serotonergic synapse;>>GABAergic synapse;>>Dopaminergic synapse;>>Long-term depression;>>Inflammatory mediator regulation of TRP channe |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Tyr657. AA range:623-672 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.