Arkadia rabbit pAb
Arkadia rabbit pAb from ELK Biotechnology Read more ›
Product Description
Arkadia rabbit pAb is an ELK Biotechnology antibody for research workflows involving Arkadia.
Background
ring finger protein 111(RNF111) Homo sapiens The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012],
Additional antibody information
| Specificity | Arkadia Polyclonal Antibody detects endogenous levels of Arkadia protein. |
|---|---|
| Validation evidence captions | Western blot analysis of RNF111 Antibody. The lane on the right is blocked with the RNF111 peptide. | Western blot analysis of the lysates from Jurkat cells using RNF111 antibody. |
Key Facts
| Target | Arkadia |
| Also known as | RNF111; E3 ubiquitin-protein ligase Arkadia; RING finger protein 111 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 110kD |
| Cellular localization | Nucleus . Cytoplasm . Nucleus, PML body . Upon TGF-beta treatment, translocates from nucleus to cytosol. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RNF111. AA range:901-950 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.