ABM98845
HLA-H rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
HLA-H rabbit pAb from ELK Biotechnology Read more ›
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Product Description
HLA-H rabbit pAb is an ELK Biotechnology antibody for research workflows involving HLA-H.
Background
This major histocompatibility complex gene represents a transcribed pseudogene, possibly derived from HLA-A. This gene displays extensive variation. [provided by RefSeq, May 2010],
Additional antibody information
| Specificity | HLA-H Polyclonal Antibody detects endogenous levels of HLA-H protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from LOVO cells, using HLAH Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HT-29 cells using HLAH antibody. |
Key Facts
Product type
Primary Antibody›
Target: HLA-H
| Target | HLA-H |
| Also known as | HLA-H; HLAH; Putative HLA class I histocompatibility antigen; alpha chain H; HLA-12.4; HLA-AR; MHC class I antigen H |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human |
| Observed band | 40kD |
| Cellular localization | Cell membrane ; Single-pass membrane protein . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HLAH. AA range:71-120 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
HLA-H
Also known as
HLA-H; HLAH; Putative HLA class I histocompatibility antigen; alpha chain H; HLA-12.4; HLA-AR; MHC class I antigen H
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human HLAH. AA range:71-120
Observed band
40kD
Cellular localization
Cell membrane ; Single-pass membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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