ABM98714
T2R43 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
T2R43 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
T2R43 rabbit pAb is an ELK Biotechnology antibody for research workflows involving T2R43.
Background
TAS2R43 belongs to the large TAS2R receptor family. TAS2Rs are expressed on the surface of taste receptor cells and mediate the perception of bitterness through a G protein-coupled second messenger pathway (Conte et al., 2002 [PubMed 12584440]). For further information on TAS2Rs, see MIM 604791.[supplied by OMIM, Mar 2009],
Additional antibody information
| Specificity | T2R43 Polyclonal Antibody detects endogenous levels of T2R43 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of TAS2R43 Antibody. The lane on the right is blocked with the TAS2R43 peptide. | Western blot analysis of the lysates from COLO205 cells using TAS2R43 antibody. |
Key Facts
Product type
Primary Antibody›
Target: T2R43
| Target | T2R43 |
| Also known as | TAS2R43; Taste receptor type 2 member 43; T2R43; Taste receptor type 2 member 52; T2R52 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 35kD |
| Cellular localization | Membrane ; Multi-pass membrane protein . Cell projection, cilium membrane . In airway epithelial cells, localizes to motile cilia. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Taste transduction |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TAS2R43. AA range:124-173 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
T2R43
Also known as
TAS2R43; Taste receptor type 2 member 43; T2R43; Taste receptor type 2 member 52; T2R52
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human TAS2R43. AA range:124-173
Observed band
35kD
Cellular localization
Membrane ; Multi-pass membrane protein . Cell projection, cilium membrane . In airway epithelial cells, localizes to motile cilia.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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